If your sample is not challenging you, then your sample might not be challenging (enough)!
ProteoPlex MacroDSF instrument
With recent technological advances in X-ray and EM technology, the specimen itself has often become the limiting factor. In EM, high-end transmisson electron microscopes and direct electron detectors are in widespread worldwide use. Astoundingly we do not yet see the same technological push and automation in the one crucial step ahead - sample preparation. Microscope and detectors cannot improve a bad specimen. Many people might say they do not have a problem with their sample and rely on conventional Thermofluor / DSF for sample optimization. We postulate that when there is no problem with the sample, the sample is too easy!
ProteoPlex MacroDSF guides you to more stable proteins and thus improves specimen quality - even and especially for complex proteins. ProteoPlex MacroDSF helps you find the ideal conditions - optimal protein concentration, pH value, salt, buffer, additive(s), etc. - to stabilize your protein. This knowledge can dramatically improve your purification results and allows high quality structure determination - e.g. your protein might regularly crystallize after MacroDSF improvement. Due to its unique biophysical algorithm, ProteoPlex MacroDSF is the only technology that can cope with protein complexes that show multi-state unfolding behavior.
Core parts of ProteoPlex MacroDSF
Suggested further reading
Chari, A.; Haselbach, D.; Kirves, J. M.; Ohmer, J.;
Paknia, E.; Fischer, N.; Ganichkin, O.; Moller, V.; Frye, J. J.; Petzold, G. et al.: ProteoPlex: Stability optimization of macromolecular complexes by sparse-matrix screening of chemical space.
Nature Methods 12 (9), S. 859-865 (2015)
Important remarks, caveats & limitations
Fig. 1: Comparison to conventional data interpretation. Comparison of melting-curve evaluation by ProteoPlex and conventional DSF. Results obtained by both methods perfectly agree (green) only for complexes that require no further optimization and are already present in a stabilized form. In early stages of a project, there is no overlap between DSF and ProteoPlex results.
Still want to read more?
Joint press release with NovAliX, 27th March 2018
Press release MPG,
7th Nov 2017:
Press release EMBL,
4th Aug 2016:
8th Aug 2016: