1 - MacroDSF analytical instrument
Recent technological advances in X-ray and EM technology have often made the specimen itself become the limiting factor. MacroDSF guides you to more stable proteins and thus improves specimen quality. The machine with its patented algorithm helps you find the ideal conditions - e.g. optimal protein concentration, pH value, salt, type of buffer, type of stabilizing additive - to stabilize your protein. This knowledge can dramatically improve your purification results and allows high quality structure determination, e.g. protein might regularly crystallize after MacroDSF improvement.
Only technology that can cope with multi-domain proteins due to unique biophysical algorithm
Success story (example):
Stability optimization of human 20S proteasome, leading to an unprecedented resolution of 1.8A and a 10x times higher specific activity than anything reported before
Answers to FAQ / Caveats:
Fig. 1: Comparison to conventional data interpretation. Comparison of melting-curve evaluation by ProteoPlex and conventional DSF. Results obtained by both methods perfectly agree (green) only for complexes that require no further optimization and are already present in a stabilized form. In early stages of a project, there is no overlap between DSF and ProteoPlex results.
Suggested further reading:
Chari, A.; Haselbach, D.; Kirves, J. M.; Ohmer, J.; Paknia, E.; Fischer, N.; Ganichkin, O.; Moller, V.; Frye, J. J.; Petzold, G. et al.: ProteoPlex: Stability optimization of macromolecular complexes by sparse-matrix screening of chemical space. Nature Methods 12 (9), S. 859-865 (2015)
Sell machine / software
Intl. patent, exclusive license from Max-Planck
Press release MPG,
7th Nov 2017:
Press release EMBL,
4th Aug 2016:
8th Aug 2016: